8/22/2023 0 Comments Legend maker upload tutorial![]() If your PDF size is larger, please use a suitable repository or discuss with the journal staff. ![]() Please note, there is a 20 MB maximum file size.All labeling and annotation should be performed without obscuring any data or background bands. Molecular weight markers should be included or indicated on the raw image, and any lanes not included in the final figure should be marked with an “X” above the lane label on the original blot/gel image. Authors should label each raw blot or gel image to clearly annotate the loading order, identity of experimental samples, method used to capture the image, and to specify which figure panel was generated from that original image.The file should be named ‘S1_raw_images’ and uploaded as a Supporting Information file or deposited at a suitable publicly-available data repository, with the dataset identifier (DOI or other form of persistent identifier) provided in the Data Availability Statement.We do not recommend compiling the images in Powerpoint as resolution can be lost. You could also use a PDF program to build a single PDF compiled from multiple annotated jpeg/tiff image files. Gimp, Photoshop) to compile and annotate the original images and then exporting/saving as a tiff file with LZW compression. ![]()
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